Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Bis-Tris Buffer (0.2 M, pH 6.5) - 250ml
The Bis-Tris Buffer is a zwitterionic agent commonly used in biological applications. It maintains a pH within 5.8 to 7.2, ideal for biological processes that require stable environments within this range. It is particularly effective in applications such as protein and nucleic acid purification, biochemical assays, and cell culture. The buffering capacity of Bis-Tris is influenced by its concentration, while its pH can be adjusted based on the ratio of Bis-Tris to its conjugate acid or base.
Research Products International Corp TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
- CAS Number: 77-86-1
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
Teknova 1X PBS Solution, pH 7.4. 500mL, Sterile 8 pack.
1X PBS Solution, pH 7.4. 500mL, Sterile 8 pack.
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt | 82207-62-3 | MFCD00043438 | 100g
HEPES potassium salt | Purity: 99.5% | Mol Wt: 273.69 | 82207-62-3 | MFCD00043438 | 100g
Medchemexpress LLC MES potassium (2-Morpholinoethanesulphonic acid potassium) | 39946-25-3 | 99.88% | 5 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer with pH range 5.5-7.0.
- Provides effective buffering capacity around pH 6.15.
- pKa value changes with temperature, requiring consideration at varying temperatures.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Exhibits poor affinity with metal ions, making it suitable for studies involving such ions.
Medchemexpress LLC Hepes, NaCl, sucrose, pH 7.2 buffer | 50 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is used to resuspend human erythrocyte ghost preparations. The buffer serves as the final resuspension medium for purified human right-side-out and inside-out erythrocyte ghosts.
- Used as a biochemical assay reagent
- Aids in the resuspension of human erythrocyte ghost preparations
- Appearance is liquid
- Color is colorless to light yellow
- Storage in pure form: -20°C for 3 years, 4°C for 2 years
- Storage in solvent: -80°C for 6 months, -20°C for 1 month
Apexbio Technology LLC MES sodium salt 71119-23-8 500mg
MES sodium salt (CAS 71119-23-8) is a buffering agent designed to maintain stable pH conditions in the range of 5 5 7 0 supporting physiological experimental environments MES sodium salt does not have a direct cellular or molecular target and is not a small-molecule inhibitor agonist or antagonist Its key role is pH stabilization in various biological and biochemical assays including enzymatic activity assays receptor pathway investigations and isolation of cellular organelles or biomolecules Experimental models include plant cell culture systems and physiological in vitro assays Based on these properties MES sodium salt holds research potential in applications such as plant cell culture controlled metabolic pathway studies and signal transduction research requiring consistent pH conditions
Research Products International Corp TRIS, ACS Grade, 5 Kilograms
TRIS, also known as tris(hydroxymethyl)aminomethane, is a chemical compound widely used in life science as a buffer.
Teknova 1X PBS Solution with 185mM Sodium Chloride, pH 7.4. 1000mL, Sterile 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 20X TBS with 0.1% Tween-20. (400mM Tris, 3M NaCl) 1000mL, Sterile. 2 pack.
3M Sodium Chloride, 400mM Tris-HCl
